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Description

Confocal Microscopy and Spectral Analysis Channel mode confocal images of BODIPY, Nile Red, and GFP fluorescence in late stage L4 animals were acquired essentially the same as previously described [21]

Briefly, mice bearing TSC2 -/- ELT3 xenograft tumors were treated every other day for 5 days with vehicle or rapamycin (1 mg/kg on day 1, 3, and 5) and tumors were harvested for metabolite extraction, as above, 3 h after the final treatment