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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

No significant difference in food intake was observed between treated and control mice in related studies, indicating that its antiobesity effect mainly depends on regulating fat metabolism rather than suppressing appetite - a unique advantage in obesity research

Effects of ultrasound and sodium lauryl sulfate on the transdermal delivery of hydrophilic permeants: ComparativeSetoJ