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Samples were first subjected to overnight digestion with concentrated sulfuric acid, followed by further digestion using a KDN-20D digestion oven (Gedana, China) at 420C for 10 minutes, until the solution became a clear blue color

Acetaminophen is a common antipyretic and analgesic, but overdose can lead to severe liver damage and acute liver failure due to the toxic metabolite NAPQI, which depletes glutathione and causes oxidative stress

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SLC7A5 acts as a mandatory exchanger to mediate the efflux of leucine and glutamine from the cell through mutual coupling with SLC1A5 [41]