glutathione agarose purification Resin optimized to yield up to 1mg of soluble GST tagged protein Glutathione Xpure Agarose Resin -

[DOI] [PubMed] [Google Scholar] Shaygannia E., Bahmani M., Zamanzad B., Rafieian-Kopaei M

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The bacterial pellet was then resuspended in 800 L of GMML liquid medium (M9 salts, 1% glycerol, 1 mM MgSO 4 and 0.1 mM CaCl 2 and 0.3 mM Leucine) containing Amp, Spe, IPTG (0.5 mM) and a specific ncAA (1 mM), This suspension was then transferred to 96-deep-well plates (2.2 mL) and incubated at 37 C, 220 rpm for 11 h, with monitoring of fluorescence intensity (excitation 485 nm and emission 515 nm) as well as OD 600 by Microplate Readers

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