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What Good HPLC Reporting Looks Like on a COA A complete HPLC section of a COA for AHK-Cu typically includes the column type and dimensions used, the mobile phase conditions, the detection wavelength (typically 215 to 220 nm for peptides, which measures the peptide bond), the retention time of the main peak (confirming it matches AHK-Cu's expected behavior), the purity percentage by peak area, and a note on any observed impurity peaks