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Single colonies obtained at the highest possible antibiotic concentration were picked and tested for successful deletion of the target gene by colony PCR using suitable flanking primers, followed by double-stranded DNA sequencing of the amplified region (Eurofins Genomics)

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In particular, mast cells signal to microglia, the resident immune cells of the brain, priming them toward a pro-inflammatory phenotype

Plant Physiol Biochem 78:1526 Cummins I, Wortley DJ, Sabbadin F, He Z, Coxon CR, Straker HE, Sellars JD, Knight K, Edwards L, Hughes D, Kaundun SS, Hutchings SJ, Steel PG, Edwards R (2013) Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds