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[DOI] [PubMed] [Google Scholar] 52.Vaysse P.J., Gardner E.L., Zukin R.S

2008 Mar;138(3): 357-362

Some extracellular-flux protocols add L-carnitine when testing long-chain fatty-acid-supported respiration.A published Seahorse XF protocol for neonatal mouse cardiomyocytes (P0P1, plated at 25,000 cells/well) used a 50 mM L-carnitine stock (stored at 20 C, stable for 612 months), diluted to a final working concentration of 0.5 mM in FAO assay medium containing palmitate-BSA (1 mM palmitate / 0.2 mM fatty-acid-free BSA) as the substrate