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After anesthesia induction with 45% isoflurane and maintenance with 2.5-3.0% isoflurane, the rats were placed in a prone position on a rat board for hair removal, disinfection, and subsequent incision.The sciatic nerve of the right hind limb, approximately 1 cm below the femur and parallel to it, was exposed by bluntly separating the subcutaneous tissues and muscles

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