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The supernatant was obtained by ultracentrifugation at 100,000 g for 1 h and then loaded onto 5 ml of Streptactin Beads 4FF (Smart-Lifesciences)

160,161 For example, HM15211, a triple agonist developed by Hanmi Pharmaceutical in South Korea that activates GLP-1R, GIPR and GCGR has entered early clinical trials for the treatment of obesity and non-alcoholic steatohepatitis (NASH)