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RABGAP1 KD cells were lifted with Accutase and plated at 1 10 6 cells/well in a six-well plate in 2 ml E8 supplemented with ROCKI

5.2 Analytical Platforms and Quality Control for GSH/GSSG Measurement Once sample integrity has been protected, the analytical platform determines selectivity, sensitivity, throughput, and whether GSH and GSSG are measured directly

It attenuates apoptosis by manipulating the ratios of Bax to Bcl-2, constraining mitochondrial membrane depolarization, and activating critical signaling pathways such as ALDH2, PI3K/Akt, and Nrf2/HO-1

The experiments with isolated mitochondria (0.5 mg/mL) were carried out at 28C with a standard reaction medium containing 15 M Ca 2+ , 10 mM Hepes, 125 mM sucrose, 2 mM K 2 HPO 4 , 65 mM KCl, 1 mM MgCl 2 , and 5 mM glutamate plus 5 mM malate (pH 7.2)