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At the structural level, we could do a protein structure alignment of the wild and mutant versions of the enzyme with and without ligands (enzyme cofactor and substrates) to know the exact interaction and how this mutation affects both the structure and activity of the enzyme

Biochemist and Nobel laureate Bruce Ames argues that the presence of a specific transporter for an exogenous molecule indicates that the human organism had regular access to ergothioneine over millions of years most likely through the consumption of mushrooms or ergothioneine-containing soil via mycorrhizal plants