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Then, the reaction mixtures were diluted with adequate amounts of 5 mM sodium phosphate buffer (pH 7.4) containing 10% (v/v) acetonitrile and filtered using a 0.45-m filter (Cosmonice filter W, Nacalai Tesque)

Measurement of ROS Formation RPE cells were seeded (2 10 4 cells/well) in black/clear-bottom 96-well plates and grown for 48 hr in RPE media containing 1% FBS, no sodium pyruvate

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Because of these reasons, creatinine-adjusted values might be good on longitudinal individual level, but may introduce errors when adjusting data on population level because of the inter-individual variability (Kestenbaum et al