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This work was funded by the National Natural Science Foundation of China (82174375), Hunan Province High-Level Health Talents 225 Project (Xiangweihan [2019]196), Hunan Province Clinical Medical Technology Innovation Guidance Project (2020SK51301), Health Research Project of Hunan Provincial Health Commission (202202124772), Hunan Provincial Natural Science Foundation (2023JJ60485), Key Project of Hunan Provincial Administration of Traditional Chinese Medicine (C2022026), Leading TCM talents in Hunan Province (Xiangweihan [2022]4), Innovative Postgraduate Projects of Hunan University of Traditional Chinese Medicine (2022CX175), Hunan Provincial Key Laboratory of Vascular Biology and Translational Medicine and Hunan Graduate Student Innovation Project (CX20220820)

To study the changes in the secondary and tertiary structure induced by GdnHCl, far-UV CD and tryptophan fluorescence studies were carried out

These findings offer potential biomarkers and therapeutic targets for clinical management

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