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Incubation was carried out for 1 h at 37 C

Homocysteine also accumulates in the blood if a mutation is present in the enzyme that converts N 5,N10-methylene-FH 4 to N 5-methyl-FH 4

To minimize fluorescence background from unreacted or non-specifically bound probes, an off-on fluorescent probe (FAsH) was developed by employing a flavylium dye as the fluorophore and a dithiarsenolane group as the arsenic-binding motif