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Description

Western Blot Analysis Cell extracts were prepared in lysis buffer containing Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 2 mM DTT, 2 mM PMSF and 1% Triton X-100 followed by ultrasonication method

Reconstituted MOTS-c must be stored at 28C and used within 28 days

Some studies [1317] have described the limitations of trying to diagnose vitamin B12 deficiency exclusively via the measurement of this concentration, and report blood methylmalonic acid (MMA) and homocysteine concentrations to be more sensitive markers capable of detecting subclinical deficiency