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Experimental Chemicals Iron (III) chloride hexahydrate (FeCl 3 .6H 2 O, 98102%), ethylene glycol ( 99%), urea ( 98%), polyvinylpyrrolidone (PVP) (MW 40,000), acetophenone (99%), sodium pyruvate ( 99%), (s)-methylbenzylamine (s-MBA) ( 98%), pyridoxal 5-phosphate hydrate (PLP) ( 98%), nicotineamide adenine dinucleotide phosphate disodium salt (NADP + ) ( 98%), nitrotetrazolium blue chloride (NBT), phenazine methosulfate (PMS) (90%), d-glucose ( 99.5%) yeast extract, tryptone digest, sodium chloride ( 99.5%), ampicillin sodium salt and phosphate buffered saline (PBS) were all purchased from Sigma Aldrich, UK