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Materials and Methods Drugs and chemicals All chemicals used were of the highest available purity and were obtained from Roth (Karlsruhe, Germany), Sigma-Aldrich (Steinheim, Germany), or Merck (Darmstadt, Germany)

GPX4 is an antioxidant enzyme that primarily reduces lipid peroxides, especially lipid hydroperoxides, into their corresponding alcohols, thus protecting cell membranes from oxidative damage [39]

Oxidative stress: impact in redox biology and medicine

Under the MASLD classification, it is also important to evaluate how GSH influences not only hepatic oxidative damage but also the broader metabolic drivers of disease