Its neuroprotective qualities open the door to fresh approaches to the treatment and recuperation of TBI
Briefly, muscles were collected, trimmed of connective tissue and fat, minced with scissors, then digested with 1.25 mg/ml of protease type XIV for 1 h at 37C
Abbreviations XSJ: Xiasangju MASH: Metabolic dysfunction associated steatohepatitis MASLD: Metabolic dysfunction-associated steatotic liver disease CDAHFD: Choline-deficient, L-amino acid-defined, high-fat diet UPLC-Q-TOFMS: Ultrahigh performance liquid chromatography with quadrupole time-of-flight mass spectrometry TCM: Traditional Chinese medicine ECM: Extracellular matrix HSC: Hepatic sellate cell -SMA: Alpha smooth muscle actin Col1a1: Collagen type I alpha 1 TGF-1: Transforming growth factor beta 1 Smad3: Mothers against decapentaplegic homolog 3 PI3K: Phosphoinositide-3 kinase AKT: Protein kinase B Hmox1: Heme oxygenase 1 Nrf2: Nuclear factor erythroid 2-related factor 2 ALT: Alanine aminotransferase AST: Aspartate aminotransferase TC: Total cholesterol TG: Triglycerides MDA: Malondialdehyde SOD: Superoxide dismutase CAT: Catalase GSH-Px: Glutathione peroxidase IL-1: Interleukin-1 IL-6: Interleukin-6 TNF-: Tumor necrosis factor- GO: Gene ontology KEGG: Kyoto encyclopedia of genes and genomes RT-qPCR: Real-time quantitative polymerase chain reaction PLS-DA: Partial least squares discriminant analysis DMs: Differential metabolites DEGs: Differentially expressed genes Msmo1: Methylsterol monooxygenase 1 Cyp51: Cytochrome P450 51 Sqle: Squalene monooxygenase PCSK9: Proprotein convertase subtilisin/kexin type 9 Batf: Basic leucine zipper ATF-Like transcription factor Ticam2: Toll-interleukin 1 receptor domain containing adaptor protein 2 Trim29: Tripartite motif-containing 29 Timp4: Tissue inhibitor of metalloproteinases 4 Thbs1: Thrombospondin 1 Mthfd2: Methylenetetrahydrofolate dehydrogenase 2 PPI: Proteinprotein interaction References Miao L, Targher G, Byrne CD, Cao Y-Y, Zheng M-H
Zhao M, Wang L, Wang M, Zhou S, Lu Y, Cui H, et al
