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Glutathione peroxidase 4, heat shock protein beta-1, and nuclear factor erythroid 2-related factor 2 function as negative regulators of ferroptosis by limiting ROS production and reducing cellular iron uptake, respectively

Results Inactivation of GSTA4 To inactivate GSTA4 in HCT116 cells, we co-transfected HCT116 cells with pLentiCRISPR-gRNA66 and pLentiCRISPR-gRNA214 ( Supplementary Table S1 ), which resulted in a 1,135 bp homozygous deletion across exons 1 and 2 in the GSTA4 gene ( Figure 1A )

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