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10.3390/children12091156 290

Patients on 7 days per month metronidazole therapy had normalized TMAO levels (PAT12-14), whereas two patients on 5 days per month metronidazole therapy had extreme TMAO elevations (PAT 17-18) suggesting that the duration of antibiotic therapy may impact microbial l-carnitine degradation (Table 1)

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Briefly, pinocytic cell loading reagent (Influx, Life Technologies) was prepared according to manufacturers instructions by heating reagent vials for 5 min to melt the PEG cap using an 80 C water bath, adding 4.7 ml of prewarmed culture medium (without serum) per vial, mixing vigorously at 37 C for 30 min to dissolve sucrose crystals, and then adding FBS (5% v/v) and sterile HEPES (10 mM)