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Archived from the original on 23 May 2021

EBioMedicine 58 , 102925 (2020)

Although significant progress has been made in buck semen cryopreservation techniques, critical challenges such as reduced post-thawing sperm motility, impaired sperm function, and oxidative stress remain unresolved [25]

After washes in PBS, cells were stained for 30 min at room temperature with a filtered Oil Red-O (Sigma) solution (0.5% Oil Red-O in isopropyl alcohol), washed again in PBS, and visualized under an inverted microscope (Olympus)