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Colon samples were flash frozen in liquid nitrogen or fixed in 4% phosphate buffered paraformaldehyde

Statistical analysis One-way ANOVA was performed on the fatty acid data using SPSS software v.23 (IBM), keeping the threshold p -value at 0.05

Immediate Steps If you suspect an allergic reaction to acetyl-L-carnitine, take these actions: Stop taking the supplement immediately to prevent worsening symptoms Take an OTC antihistamine to reduce mild allergic symptoms Seek emergency care if experiencing difficulty breathing, swelling of the face or throat, or severe symptoms Document your symptoms to share with your healthcare provider Sublingual Immunotherapy While sublingual immunotherapy is highly effective for environmental and certain food allergies, it is not currently a standard treatment for supplement allergies like acetyl-L-carnitine

Frequency: Typically once a week for the initial course, followed by bi-weekly or monthly maintenance