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N Engl J Med 359: 26192620

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After 4-day culture, organoids were dissociated in organoid dissociation solution (bioGenous, E238001) for 2 min at 37 C according to the manufacturers protocol, centrifuged at 68 g for 2 min, resuspended in organoid culture medium, and cultured as described above for organoids passaging

Moreover, microbial products, cytokines, and other immune molecules released under the influence of the GM may further influence the BBBs integrity, alter BBB transport rates, and promote the release of neuroimmune molecules from the cells of the BBB (262)