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Differential expression of histamine receptors in the bladder wall tissues of patients with bladder pain syndrome/interstitial cystitis significance in the responsiveness to antihistamine treatment and disease symptoms

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Dimethyl sulfoxide diluted in a 0.01M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, Vt.)