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The pooled and concentrated protein was applied to a 16/60, 75 pg, Superdex size-exclusion chromatography column (Cytiva, Freiburg, Germany) in 50 mM Tris/HCl, pH 8.0, 500 mM NaCl, 1 mM DTT at 4 C

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Ayurveda contains a particular group of Rasayana herbs, called vajikarana , that deals with nourishment and stimulation of sexual tissues, improves male reproductive vitality, and deals with oxidative stress via antioxidant mechanism