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After 4 days, the TS2/16 scFv-Fc was captured from the conditioned media with CaptivA HF protein-A affinity resin (Repligen, CA-PRI-0100) and eluted with IgG elution buffer (Thermo Scientific) containing 300 mM NaCl and neutralized with 100 mM Tris pH 8.0 to reach eventually pH 7.0

Vitiello MV, Wilkinson CW, Merriam GR, Moe KE, Prinz PN, Ralph DD, Colasurdo EA, Schwartz RS

Interestingly, type I IFN targets also human neutrophils, for instance by prolonging their survival 16 , or by inducing the expression of CXCL10 mRNA 17 and the production of biologically active TNF-related apoptosis-inducing ligand (TRAIL) 18

Stop immediately if you notice black stools or severe pain lasting over 24 hours, as these signal serious complications