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Available RCTs comparing oral versus intravenous acetaminophen have detected no difference in efficacy

Abbreviations CSE: Cigarette smoke extract TNF-: Tumor necrosis factor- EMT: Epithelial-mesenchymal transformation COPD: Chronic obstructive pulmonary disease CS: Cigarette smoke NAC: N -Acetylcysteine VWF: Von Willebrand factor MAPK: Mitogen-activated protein kinase FEV1: Forced expiratory volume in 1 FVC: Forced vital capacity PMSF: Phenylmethylsulphonyl fluoride ECL: Enhanced chemiluminescence SABC: Streptavidin biotin peroxidase complex ACK: Ammonium chloride-potassium FITC: Fluorescein isothiocyanate NC: Negative control DMEM: Dulbecco's modified Eagle's medium oe: Overexpression sh: Short hairpin RNA SD: SpragueDawley BCA: Bicinchoninic acid PEF: Peak expiratory flow HE: Hematoxylineosin RT-qPCR: Reverse transcription quantitative polymerase chain reaction GAPDH: Glyceraldehyde-3-phosphate dehydrogenase RIPA: Radio-immunoprecipitation assay SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis PVDF: Polyvinylidene fluoride ANOVA: Analysis of variance References Armstrong J, Harbron C, Lea S, Booth G, Cadden P, Wreggett KA, et al

The beads were washed three times for 5 min each with low-salt wash buffer containing 20 mM Tris-HCl, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl, and 0.1% SDS

The procedure typically takes 10 to 15 minutes