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GGT activity was reported to be particularly high in tissues with secretory and absorptive function such as kidney, biliary system, intestine and epididymis and the enzyme activity is greatest in the ductal luminal surface of these tissues (3)

Regulation of TIMP-1 expression involves the activation of transcription factors including AP-1, Sp1, STAT, and Pea3/Ets families, as well as signaling pathways such as the ERK1/2 pathway [51]-[53]

In conclusion, although the limited number of our validation cohort, these results suggested the same characteristics in the public cohort and Beijing cohort based on the FRGs classification