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After RNA quantification using the Nanodrop 2000 (Thermo Fisher Scientific, USA), 1 g of total RNA was reverse transcribed in a total volume of 20 l using the PrimeScript RT reagent kit (Takara Bio, Tokyo, Japan)

Glycolytic intermediate 3-PG is converted to serine through a three-step enzymatic cascade, generating metabolite intermediates for lipid, nucleic acid, protein, and antioxidant biosynthesis

CD47 proteins rich on the red cell membrane ensure red cell membrane-coated systems can circulate almost 100120 days without being cleared by macrophages

Particularly, in SF3B1-MDS, there is a significant decrease in lymphocyte percentages and an increase in erythroid percentages