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For example, if the nucleotide sequence of the antibody is known, a polynucleotide encoding the antibody may be assembled from chemically synthesized oligonucleotides (e.g., as described in Kutmeier et al., BioTechniques 17:242 (1994)), which, briefly, involves the synthesis of overlapping oligonucleotides containing portions of the sequence encoding the antibody, annealing and ligating of those oligonucleotides, and then amplification of the ligated oligonucleotides by PCR

This suggests that precise dosing may be less critical than consistent daily administration over an adequate duration

And above all, is COPD a risk factor for CVD or the opposite

The gradient is centrifuged at 400 g for 15 min to isolate a low density fraction