CRISPR-Test Cas9 and dCas9 Activity Test Kits Type:CRISPRaTest dCas9-Activator Assay Kit (to test 5-15 cell lines) Fig 1: Mean clonotype counts

Fig 1: Mean clonotype counts for four TCR chains from different DNA sample inputs

which relies on cell death induced by disruption of a gene required for viability

This Assay is similar to the CRISPRuTest Cas9 Fluorescent Reporter Assay in that both use the disappearance of a fluorescent marker to assess Cas9 activity

streamlined protocol: Sort cells into an indexed 96-well plate with RT primers

CRISPR-Test Cas9 and dCas9 Activity Test Kits Type:CRISPRaTest dCas9-Activator Assay Kit (to test 5-15 cell lines) Fig 1: Mean clonotype countsCellecta's CRISPR Functional Cas9 and dCas9 Activity Test Kits measure the functional activity of Cas9 nuclease, dCas9 repressor, or dCas9 activator by measuring loss of GFP in any mammalian cell line. 1. a. CRISPRuTest Functional Cas9 Activity Assay Kit (Catalog # CRUTEST) can be used to measure the Cas9 nuclease activity in any vertebrate cell system. The CRISPR system uses a target single guide RNA molecule (sgRNA) to target and cut a specific