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Patients may feel a brief sting at the injection site, but the discomfort is usually minimal and short-lived

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TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions

Spirulina Spirulina can potentially increase the activation of macrophages and NK-cells, improving immune response against foreign invaders, and of T-cells functions, promoting their activation and proliferation