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The abundances of Coprobacillus , Eggerthella , and Blautia were key differentiators

The Baltimore Sun

Sterol composition of black cumin (Nigella sativa L.) and Aleppo pine (Pinus halepensis Mill.) seed oils

For Cyss depletion, cells were washed with phosphate-buffered saline solution (PBS), pH 7.4, and incubated for the indicated times in DMEM, high glucose, no glutamine, no methionine, no cystine (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS), 0.2 mM l-Methionine, 100 g/ml streptomycin, 100 U/ml penicillin, and 2 mM l-glutamine