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Description

Myosin is mainly involved in the regulation of TJ assembly and tone (129), while actin binds to the cytoplasmic scaffold proteins (130)

Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded, while anti-MBP (Rat, BioRad MCA4095,1:1000) was used to detect MBP