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A successful pH-sensitive mutant would exhibit a K_D comparable to the wild-type antibody at neutral pH to ensure cell surface binding, but a significantly weakened K_D (markedly increased k_off rate) at acidic pH to facilitate rapid dissociation in the lysosome and enable efficient receptor recycling [76]

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Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography

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