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Tumor tissues were excised from anesthetized mice and homogenized in HEPES-buffered saline containing 10% glycerol, 1 mM EDTA, 2 mM EGTA, 1 mM DTT, 5 mM Na 3 VO 4 , 5 mM NaF, 1 mM AEBSF, aprotinin (5 g/ml), and leupeptin (5 g/ml) using a Dounce homogenizer

[5] Cellular location of the pyruvate dehydrogenase complex In eukaryotes, the pyruvate dehydrogenase complex, like the enzymes of the citric acid cycle and fatty acid oxidation, is located in the mitochondrion , where it is associated with the surface of the inner membrane facing the matrix

Mitophagy, a type of autophagy , is a kind of cellular recycling system that clears damaged mitochondria before they can accumulate and cause cellular dysfunction

Additionally, patients in the NAC group experienced significant improvements in Parkinson's symptoms