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According to the NIH, "the most common causes of constipation are poor diet and lack of exercise." Regarding diet, it's caused by "a diet low in fiber or a diet high in fats, such as cheese, eggs, and meats." Aside from more fiber, less fats and increased activity, the NIH recommends plenty of water, juice or other liquids free of alcohol and caffeine, which may worsen constipation

The supernatant was filtered through a 0.45 m syringe filter into a 15 mL centrifuge tube collecting about 3 mL solution

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