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Mechanisms of CVD and CKD in patients with diabetes Vascular damages of ASCVD in patients with diabetes are characterized by endothelial and vascular smooth muscle cell dysfunction [12]

Incubate cells at 4 C for 10 min followed by centrifugation for 10 min at 14,000 rpm and 4 C

2.5.2 LCMS/MS data-dependent acquisition Digested samples were analyzed by nano HPLC using an EASY-nLC 1,000 chromatograph coupled to a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific, Pittsburgh, PA, United States), using an EASY-Spray Accucore (P/N ES801) Thermo Scientific reverse phase column coupled to an EASY-SPRAY Electro Spray ionizer (Thermo Fisher Scientific, Pittsburgh, PA, United States), with Spray voltage between 2.53.5 kV