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RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)

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The Ridpath is still here with one guy putting his own money into it to make sure people have resources for food and shelter. The 15 years it took to turn the Ridpath from abandoned hotel into apartments cost an estimated $30 million and included a complex web of condo sales, foreclosures and intervention by the city of Spokane in 2011 after transients began squatting at the property