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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h

[22] Acquisition by CVS [edit] On August 12, 2008, CVS announced that it would acquire the Longs Drugs chain of 521 stores to expand its presence on the West Coast, and gain access to the Northern California and Hawaii markets

Eligible purchases typically include: Made-To-Order meals Coffee and fountain drinks Snacks Packaged beverages Merchandise Online orders Usually excluded: Fuel purchases Alcohol Tobacco Lottery tickets Gift cards Some regulated products Need menu ideas

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