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5-EU click reaction was performed in buffer containing 100 mM Tris-HCl (pH 8.5), 2 mM CuSO 4 , 100 mM sodium ascorbate, and 5 M Alexa Fluor 488 Azide (Thermo Fisher Scientific, A10266) for 30 min in the dark followed by two washes with 1% (w/v) BSA in PBS

Does the type of bacteriostatic water affect how much I need to add

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Organizations and companies like the NHL and various Ice Rink manufactures have gone to greater measures to preserve the activities that need these facilities to operate on by starting in the direction of a greener future