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H 2 S Reactivity H 2 S has strong nucleophilic properties that allow it to react with electrophilic species, whereas sulfur is a good reductant and will react with oxidants

The resulting sections were mildly fixed with 2% paraformaldehyde (VWR Life Science) for 10 min, permeabilized by incubation in 0.05% Triton X-100 for 10 min and blocked with 5% BSA for 1 h at room temperature

Owing to potential interference from cfDNA of non-tumor origin, which may be released into the urine from the systemic circulation, the concentration of ctDNA in urine becomes diluted, resulting in reduced levels of detectable ctDNA

The patient harboured the following genetic point mutations: KRAS (G61H), SMAD4 (Asn107Lys), BRCA2 (Arg2034Cys), and PRSS (Cys171Tyr)