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Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]

The usefulness of toxicogenomics for predicting acute skin irritation on in vitro reconstructed human epidermis

NF-B suppression by the deubiquitinating enzyme Cezanne: a novel negative feedback loop in pro-inflammatory signaling