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Statistical methods All statistical analyses were performed, and all data figures were created using PRISM GraphPad version 9.1.2 (La Jolla, USA) unless otherwise specified

Over the past few decades, there has been a growing interest in understanding the neurobiological underpinnings of glutamatergic dysfunctions in the pathogenesis of depressive disorders and the development of new pharmacological and non-pharmacological treatment options

Methods: H69 cholangiocytes, THP1 monocyte cell line and cholangiopathy mouse model (0.1% diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate [DDC] diet feeding x14d) were used

In this regard, calpain activity can be elevated by a sustained increase in free calcium in the cytosol and/or a decrease in calpastatin levels (127)