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Key features Readily assessable HMGB1::Lucia reporter activity Convenient readout using QUANTI-Luc 4 Lucia/Gaussia Highly sensitive to ferroptosis inducers Stability guaranteed for 20 passages Applications Therapeutic development Inhibitor and activator screening Release assay Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions)

Cells were tested routinely for mycoplasma (Bulldog Bio Inc.) e-Myco PLUS Mycoplasma PCR Detection Kit (Bulldog Bio Inc)

Discussion FSEC-TS assays for fluorescently tagged membrane proteins are best performed at temperatures where the tags are largely unaffected (~25% drop of fluorescence) so that the decrease of fluorescence can be largely attributed to the aggregation/precipitation of protein of interest (POI)

Li W, Li Z, Yang J, Ye Q