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After sorting, neutrophils were centrifuged at 500 g at 4 C and resuspended in RPMI-1640 medium with 10% FBS for subsequent culture, stimulation and functional assays

2009;4(3):e4715

Similarly, the application of personalized medicineguided by genomic and transcriptomic profilingrelies on reliable biomarkers, which are still under development for many resistance mechanisms