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Samples were then digested with trypsin (11 L of 0.5 g/L per sample, resuspended in trypsin resuspension buffer containing 20 mM CaCl 2

In addition to the high content of readily oxidizable molecules like catecholamines, and polyunsaturated fatty acids, and relatively lower antioxidant levels compared to other organs, these factors make the brain more vulnerable to oxidative stress