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This upregulation was also reflected in increased GSH levels (Fig

Zhang J, Wang E, Zhang L, Zhou B

The intracellular concentration of glutathione (GSH) is in the millimolar range and the extracellular concentration is in the micromolar range

Nrf2, upon translocating to the nucleus, binds to antioxidant response elements (AREs) located in the promoter regions of various genes ( Figure 3 ) ( Figure 3 4.1 Bioactive compounds boost antioxidant and anti-inflammatory responses by activating Nrf2/KEAP1 signaling to combat mastitis: in vitro evidence It has been well established through in vitro experiments that bioactive compounds can enhance the activation of the Nrf2/KEAP1 signaling pathway