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The cells were then washed using MACS buffer (0.5% BSA + 2mM EDTA in PBS) followed by staining with different clones of anti-CD38: LS198-4-2 (Beckman Coulter), JK36 (Beckman Coulter) and HIT2 (BD Biosciences) for 10 mins in the dark at room temperature

The second medicine in the injection is Methylcobalamin which is known as vitamin B12

AOD9604 and Energy Balance Research In obese ob/ob and lean C57BL/6J mice treated for 14 days via mini-osmotic pumps, AOD9604 significantly reduced body weight gain in obese mice, associated with increased in vivo fat oxidation and increased plasma glycerol levels as an index of enhanced lipolysis without the hyperglycaemia or reduced insulin secretion associated with intact hGH treatment