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Accordingly, a conversion efficiency of 99.32% for the fatty acid methyl esters (FAME) was obtained underneath the optimum condition, the temperature of 300 C, extraction time of 30 min, methanol-to-dry Spirulina of 8 mL/g, hexane-to-dry Spirulina of 4 mL/g, as well as moisture content of 40% (Shirazi et al